lab plasticware

Before each use of lab plasticware the rotor, strictly check whether there is any foreign matter in the hole to maintain the balance during centrifugation. Leave at least 30 cm of space around the centrifuge. Use a centrifuge tube with a screw cap (Figure 3) and wait 20 minutes after the centrifugation After opening the centrifuge lid, wait another 20 minutes to open the rotor lid and take out the sample. Use after drying.. Centrifuges produce aerosols during high-speed operation (Figure 1). 1

The main reasons for the accident ① When the centrifuge was balanced, the balance of the centrifuge tube placed symmetrically exceeded 0. ③ When using, place the centrifugal items symmetrically and with equal mass. The Versati series has strong versatility to meet all your application needs and is suitable for a variety of experimental consumables such as universal tubes, row tubes, and microplates. Aerosols contain pathogenic microorganisms, which cause great damage to the environment, the human respiratory system, digestive system, and nervous system. After each operation, a record of the use should be made, and the performance of the centrifuge should be checked regularly. ②

When the centrifuge tube is loaded, the centrifuge tube cap is not tightened, and the high vacuum state in the centrifuge chamber caused the centrifuge tube to rupture and the sample liquid to overflow during the centrifugation process, which caused the rotor to be unbalanced and the shaft to be bent or broken.5% or 75% alcohol and then wiped with water.

The rotor must be kept dry and clean to avoid collision and abrasion. The unbroken covered tube disinfects the surface of the contaminated container with a disinfectant containing chlorine; the internal surface of the centrifuge can be wiped twice with a chlorine-containing disinfectant with an effective chlorine concentration of 0. ① Centrifuge installation: Choose a suitable place to place according to the performance of the centrifuge. ④ Improper use and improper disinfection of the rubber seal ring and rotor cover rubber seal ring in the centrifuge tube cap. 

Megosztás a facebookon

laboratory manufacturers

It has a detoxifying effect and can relieve the toxicity of fatty acids, heavy metals and certain proteases. Bovine serum is one of the traditional Chinese medicine components in cell culture medium. The serum contains various plasma laboratory manufacturers proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc.Serum is a very complex mixture formed by the removal of fibrin from plasma.

The shelf life of serum is only one year, and the efficacy of serum is different in different storage periods. Therefore, when newly purchased serum or when starting a major test, screening of bovine serum should be performed first. 3. The composition and content of serum often vary with the sex, age, physiological conditions and nutritional conditions of the blood donor. The reason why scientific researchers choose to use bovine serum for cell experiments is as follows: 1.

In the cryo rack, turbulence is rare and can only be found during pathophysiology.1067 / mva. In the body, uneven laminar flow occurs at the branching points of blood vessels. Experimentally, this type of flow can be simulated by using a flow with a periodically varying flow rate and a constant flow direction.

When performing PCR: * Please make sure you are not using too much starting DNA or too high concentration of primers, and do not add too much Mg ++ * Please make sure you use the proper annealing temperature * Please make sure you are not using too much DNA polymerase IV. Generacer1

A few more clones were tested using upstream and downstream primers. The target gene is too long to be suitable for reverse transcription. 8. Care must be taken to ensure that RNA is not degraded. * It is recommended to add control RNA in the test. Due to the secondary structure of the RNA template, such as circular results, GSP may not be able to anneal to the template; or SSII reverse transcriptase cannot be effectively extended from this primer.

Megosztás a facebookon

The amount of freezing cryo tube loaded is not enough to increase the amount of DNA loaded. Ethidium bromide (EB) used in electrophoresis is moderately toxic and highly carcinogenic. Do not heat before electrophoresis. Using agar gel as a support, the charge effect and molecular sieve effect of DNA molecules in an electric field are used to achieve the purpose of separating mixtures. Nucleic acid electrophoresis usually uses agarose gel and polyacrylamide gel. Therefore, in order to obtain a more realistic electrophoresis result, you can soak and stain with EB solution at 0.

The greater the electric field strength, the faster the movement of the charged particles. DNA bands with similar molecular size are not easy to distinguish and increase the electrophoresis time. Add TBE running buffer to the tank until the liquid surface covers the gel surface. When the BE-DNA complex is irradiated with ultraviolet light, the position of the nucleic acid is indicated by luminescence. DNA denaturation. Polyacrylamide gel electrophoresis is more sensitive than agarose electrophoresis.

Each section is equivalent to 1% of the total column bed volume. The filler in the chromatography column is some inert porous network structure materials, mostly cross-linked glycans, so that the materials in the protein mixture are separated according to different molecular sizes. 9. 2. The output tube was opened to allow the sample to flow into the bed and the inner wall of the column above the gel was rinsed with buffer.

Pre-swelled gels are placed in a Buchner funnel. 6. Pour the gel suspension into the column to the set height along a glass rod placed along the inner wall of the column, then carefully add a layer of buffer 1 cm high to the top of the gel, and connect the buffer storage container Remove the syringe blocking the column output tube, and wash the column with 2 to 3 times the volume of the column bed.02% sodium azide. Collect the effluent in sections.Gel filtration chromatography (gel filtration chromatography), also known as exclusion chromatography or molecular sieve method, is mainly based on the size and shape of the protein, that is, the quality of the protein for separation and purification

Megosztás a facebookon

If the temperature is too low, the tissue block will be too hard, the section will be fragmented, and there will be terrace-like thickness unevenness or voids; on the contrary, if the temperature is too high, the tissue block will not be hard enough, and the section will not be easily formed or wrinkled. Data Figure 1 is for reference. ④ When slicing, if you find that the frozen tissue is excessively frozen, remove the frozen tissue together with the supporter, leave it at room temperature for a while, and then perform sectioning, or breathe in your mouth, or press the tissue block with your thumb to soften the tissue Slice again. Also, increase the freezing point. ⑤

The slides used for attaching the slices should not be stored in a frozen place, just at room temperature. Because when attaching the slide, there is a temperature difference between the slide taken from the room temperature and the slide in the freezer.

When the slide with a higher temperature is attached with the lower temperature, the temperature between the two substances is different. The difference is that when they collide together, the molecules are transferred to each other and an adsorption force is generated, which makes the slice and the glass slide firmly adhere to each other. If refrigerated slides are used to attach the sections, the above phenomenon does not occur due to the same temperature. Beijing Bioway Biotechnology Co., Ltd.

has Biolog microbiological identification system, ultra-low temperature refrigerators, biological safety cabinets and other instruments and equipment for routine molecular experimental research on microbial separation and identification. To meet the needs of China's life science research, biotechnology innovation and industrial development, it actively faces the society and even abroad to collect and provide microbial strain resources. On the premise of ensuring biosafety and protecting intellectual property rights, microbial species resources, genetic resources, information resources and professional technical services are provided for industrial and agricultural production, health and health, environmental protection, scientific research and education. http://www.biobw.com/

Megosztás a facebookon