The amount of freezing cryo tube loaded is not enough to increase the amount of DNA loaded. Ethidium bromide (EB) used in electrophoresis is moderately toxic and highly carcinogenic. Do not heat before electrophoresis. Using agar gel as a support, the charge effect and molecular sieve effect of DNA molecules in an electric field are used to achieve the purpose of separating mixtures. Nucleic acid electrophoresis usually uses agarose gel and polyacrylamide gel. Therefore, in order to obtain a more realistic electrophoresis result, you can soak and stain with EB solution at 0.

The greater the electric field strength, the faster the movement of the charged particles. DNA bands with similar molecular size are not easy to distinguish and increase the electrophoresis time. Add TBE running buffer to the tank until the liquid surface covers the gel surface. When the BE-DNA complex is irradiated with ultraviolet light, the position of the nucleic acid is indicated by luminescence. DNA denaturation. Polyacrylamide gel electrophoresis is more sensitive than agarose electrophoresis.

Each section is equivalent to 1% of the total column bed volume. The filler in the chromatography column is some inert porous network structure materials, mostly cross-linked glycans, so that the materials in the protein mixture are separated according to different molecular sizes. 9. 2. The output tube was opened to allow the sample to flow into the bed and the inner wall of the column above the gel was rinsed with buffer.

Pre-swelled gels are placed in a Buchner funnel. 6. Pour the gel suspension into the column to the set height along a glass rod placed along the inner wall of the column, then carefully add a layer of buffer 1 cm high to the top of the gel, and connect the buffer storage container Remove the syringe blocking the column output tube, and wash the column with 2 to 3 times the volume of the column bed.02% sodium azide. Collect the effluent in sections.Gel filtration chromatography (gel filtration chromatography), also known as exclusion chromatography or molecular sieve method, is mainly based on the size and shape of the protein, that is, the quality of the protein for separation and purification

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