If the temperature is too low, the tissue block will be too hard, the section will be fragmented, and there will be terrace-like thickness unevenness or voids; on the contrary, if the temperature is too high, the tissue block will not be hard enough, and the section will not be easily formed or wrinkled. Data Figure 1 is for reference. ④ When slicing, if you find that the frozen tissue is excessively frozen, remove the frozen tissue together with the supporter, leave it at room temperature for a while, and then perform sectioning, or breathe in your mouth, or press the tissue block with your thumb to soften the tissue Slice again. Also, increase the freezing point. ⑤

The slides used for attaching the slices should not be stored in a frozen place, just at room temperature. Because when attaching the slide, there is a temperature difference between the slide taken from the room temperature and the slide in the freezer.

When the slide with a higher temperature is attached with the lower temperature, the temperature between the two substances is different. The difference is that when they collide together, the molecules are transferred to each other and an adsorption force is generated, which makes the slice and the glass slide firmly adhere to each other. If refrigerated slides are used to attach the sections, the above phenomenon does not occur due to the same temperature. Beijing Bioway Biotechnology Co., Ltd.

has Biolog microbiological identification system, ultra-low temperature refrigerators, biological safety cabinets and other instruments and equipment for routine molecular experimental research on microbial separation and identification. To meet the needs of China's life science research, biotechnology innovation and industrial development, it actively faces the society and even abroad to collect and provide microbial strain resources. On the premise of ensuring biosafety and protecting intellectual property rights, microbial species resources, genetic resources, information resources and professional technical services are provided for industrial and agricultural production, health and health, environmental protection, scientific research and education. http://www.biobw.com/

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