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It has a detoxifying effect and can relieve the toxicity of fatty acids, heavy metals and certain proteases. Bovine serum is one of the traditional Chinese medicine components in cell culture medium. The serum contains various plasma laboratory manufacturers proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc.Serum is a very complex mixture formed by the removal of fibrin from plasma.
The shelf life of serum is only one year, and the efficacy of serum is different in different storage periods. Therefore, when newly purchased serum or when starting a major test, screening of bovine serum should be performed first. 3. The composition and content of serum often vary with the sex, age, physiological conditions and nutritional conditions of the blood donor. The reason why scientific researchers choose to use bovine serum for cell experiments is as follows: 1.
In the cryo rack, turbulence is rare and can only be found during pathophysiology.1067 / mva. In the body, uneven laminar flow occurs at the branching points of blood vessels. Experimentally, this type of flow can be simulated by using a flow with a periodically varying flow rate and a constant flow direction.
When performing PCR: * Please make sure you are not using too much starting DNA or too high concentration of primers, and do not add too much Mg ++ * Please make sure you use the proper annealing temperature * Please make sure you are not using too much DNA polymerase IV. Generacer1
A few more clones were tested using upstream and downstream primers. The target gene is too long to be suitable for reverse transcription. 8. Care must be taken to ensure that RNA is not degraded. * It is recommended to add control RNA in the test. Due to the secondary structure of the RNA template, such as circular results, GSP may not be able to anneal to the template; or SSII reverse transcriptase cannot be effectively extended from this primer.
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